Current Vacancies

PostDoc (36 months) position available! Application deadline: 18 Jan. 2018 (see below)

New Phd studentship is available funded by The Leverhulme Trust. Students worldwide are eligible to apply! See below for more information.

For more information, see down below or contact us: t.mock@uea.ac.uk; c.van-oosterhout@uea.ac.uk

1) PostDoc position:

FACULTY OF SCIENCE, SCHOOL OF ENVIRONMENTAL SCIENCES

Senior Research Associate ● Ref: RA1483

£32,548 to £38,833 per annum

 UEA’s School of Environmental Sciences has become one of the largest and most fully developed interdisciplinary institutions of its kind in Europe. We have an opportunity for a researcher to work on a NERC funded project which will examine how polar diatoms respond to changes in their natural environment by studying keystone species from the Arctic Ocean using evolutionary genetics, environmental molecular biology, experimental evolution and ecology approaches (see Mock et al. 2017, Nature, DOI: 10.1038/nature20803). You will be a postdoctoral scientist from the field of molecular microalgal research and will conduct long-term and high-throughput growth experiments with polar diatoms under relevant environmental conditions in the laboratory. You will have a relevant PhD (or equivalent qualification), experience in molecular biologist with laboratory experience in the field of sequence-led research with microalgae, and be able to fulfil all essential areas of the person specification.

This full time post is available from 1 February 2018, or very soon thereafter, for a fixed term period of 35 months.

Closing Date:  18 January 2018.

To apply for this vacancy, please follow the online instructions at: www.uea.ac.uk/hr/vacancies/ or push 'How to apply'.

4) Molecular underpinnings of adaptive evolution of diatoms in polar oceans  (MOCKTU18LEV)

Project Description

The overarching aim of this PhD studentship is to apply CRISPR-Cas mediated genome editing in the polar diatom Fragilariopsis cylindrus to identify (epi)genes and their alleles, which underpin the evolution and adaptation of this model polar phytoplankton species. Recent and ongoing research has identified a number of genes and allelic variants in polar diatoms that are highly expressed under stressful environmental conditions (Mock et al. 2017 Nature, DOI: 10.1038/nature20803 https://www.nature.com/articles/nature20803). We hypothesise that these genetic variants enable phenotypic plasticity and an adaptive response to changing conditions due to global warming). Our group pioneered the application of CRISPR-Cas for genome editing in diatoms. The student will extend genome editing to polar eukaryotic organisms, which have not been subjected to genome editing yet. The student will be working together with an experienced postdoctoral research associate (PDRA), who developed the CRISPR-Cas protocol for diatoms and the protocol to transform F. cylindrus. The student will knockout genes identified by the PDRA through comparative genomics and experimental evolution. The knockout cell lines will be subjected to competition experiments with wild-type cells. Furthermore, the student will physiologically and biochemically characterize the mutant cell lines according to the predicted functions of the mutated genes. Thus, this studentship is at the cutting edge of polar research as it will a) enable to apply the latest genome editing tools to address fundamental questions about the evolution of key polar primary producers and their ability to respond to a rapidly changing polar environment, and b) it will go beyond mere statistical inference by providing an experimental validation of the cause-effect relationship between (epi)genes and adaptation to environmental change in one of the most threatened ecosystems on our planet.

For more information on the supervisor for this project, please go here: http://mocklab.com/people/

The type of programme: PhD

The start date of the project: April 2018

The mode of study: full-time

First degree subjects: Biological Sciences, including medicine. Minimum standard entry requirement is 2:1.

Funding Notes

This studentship is funded by the Leverhulme for three years. Funding is available to UK/EU applicants and comprises of payment of tuition fees and a maintenance stipend at RCUK rates. Overseas applicants may apply but they are required to pay the difference between home/EU and overseas tuition fees. (in 2017/18 the difference is £13,805 but fees are subject to an annual increase)

References

1) Belshaw N., Grouneva I., Aram L., Gal A., Hopes A., Mock T. (2017) Efficient CRISPR/Cas-mediated homologous recombination in the model diatom Thalassiosira pseudonana. bioRxiv
(https://www.biorxiv.org/content/early/2017/11/09/215582)
2) Hopes A., Nekrasov V., Belshaw N., Grouneva I., Kamoun S., Mock T. (2017) Genome editing in diatoms using CRISPR-Cas to induce precise bi-allelic deletions. Bio-Protocol
(DOI: 10.21769/BioProtoc.2625, http://www.bio-protocol.org/e2625)
3) Hopes A., Nekrasov V., Kamoun S., Mock T. (2016) Editing of the urease gene by CRISPR-Cas in the diatom Thalassiosira pseudonana. Plant Methods
(DOI: 10.1186/s13007-016-0148-0, https://plantmethods.biomedcentral.com/articles/10.1186/s13007-016-0148-0)
4) Paajanen P., Strauss J., van Oosterhout C., McMullan M., Clark D.M., Mock T. (2017) Building a locally diploid genome and transcriptome of the diatom Fragilariopsis cylindrus. Scientific Data
(DOI: 10.33810.1038/sdata.2017.149, https://www.nature.com/articles/sdata2017149)
5) Mock T., Otillar R.P., Strauss J., McMullan M., Paajanen P., Schmutz J., Salamov A., Sanges R., Toseland A., Ward B.J., Allen A.E., Dupont C.L., Frickenhaus S., Maumus F., Veluchamy A., Wu T., Barry K.W., Falciatore A., Ferrante M.I., Fortunato A.E., Glöckner G., Gruber A., Hipkin R., Janech M.G., Kroth P.G., Leese F., Lindquist E.A., Lyon B.R., Martin J., Mayer C., Parker M., Quesneville H., Raymond J.A., Uhlig C., Valas R.E., Valentin K.U. Worden A.Z., Armbrust E.V., Clark M.D., Bowler C., Green B.R. Moulton V., Van Oosterhout C., Grigoriev I.V. (2017) Evolutionary genomics of the cold-adapted diatom Fragilariopsis cylindrus. Nature
(DOI: 10.1038/nature20803, https://www.nature.com/articles/nature20803)